![]() ![]() shRNA constructions based on pre-defined scaffolds are also automated. You can assemble fragments, obtained by PCR, adaptor/shRNA synthesis or simply by graphically selecting fragments between restriction sites. Just select, blunt if you need, and click the Ligate button. An additional interface allows easy Gateway(tm) cloning for both BP and LR reactions. During all these sub-cloning steps, Features present in plasmids and insert will be properly inherited.įinally, Serial Cloner provides an interface to align two sequences using a local algorithm or the BLAST2Seq NCBI server and extract a consensus. Features of the aligned sequences are displayed. Perera, Julián.A virtual cutter, a web browser with instant import of NCBI/EMBL entries and a silent restriction map generator are also provided. Recombinant DNA : genes and genomes - a short course, 2007Ħ) J. ![]() Twyman Principles of gene manipulation and genomics /, SEVENTH EDITION, eBook | 2006ĥ) H. An Introduction to genetic engineering eBook | 2008Ĥ) S. Brown eBook | 2016Ģ) MOLECULAR BIOTECHNOLOGY, PRINCIPLES AND APPLICATIONS OF RECOMBINANT DNAHarris, Bernadette Harris, Bernadette eBook | 2018ģ)Nicholl, Desmond S. Two of the problem sessions will be held in the computer room.Īnnotation: Within the schedule set by the centre or degree programme, 15 minutes of one class will be reserved for students to evaluate their lecturers and their courses or modules through questionnaires.ġ) Gene Cloning and DNA Analysis : An IntroductionT. At the beginning of the semester, a dossier will be delivered through the Virtual Campus with the problem statements, which will be solved by the teacher in a reasoned way and, if necessary, complementing part of the subjects explained in the theory classes. Students will attend scheduled sessions for their group. For these sessions, the theory group will be divided into two subgroups (A and B), the lists of which will be made public at the beginning of the course. There will be 8 problem sessions per group. The teacher will explain the content of the syllabus with the support of audiovisual material that will be available to students in the Virtual Campus of the subject The activities consist of theory and problem sessions. "Two-hybrid" method for detecting protein-protein interactions. Expressions of recombinant proteinsin yeast. Site-directed mutagenesis vs Molecular evolution (Phage display). Optimization of recombinant protein expression. Unit 7. Expression of recombinant proteins in E. " Genomic Walking" and/or obtention of probe (reverse PCR). Vectors used in genomic llibraries: Lambda, Cosmids, BACS. Strategies for obtaining libraries for genomic sequencimg. Construction and screening of genomic llibraries versus high throughput genomic sequence. Unit 6. Libraries for genomic sequencing. RT-PCR / RNA-seq as an alternative to cDNA libraries. Main vectors used in the construction of cDNA libraries. Strategies for the construction of libraries, concept of abundance and complexity of mRNA. Unit 5. Libraries of cDNA versus RT-PCR/RNA-seq. Specific vectors by alternative cloning systems: recombination integration systems, topoisomerase-based cloning systems. Characteristics of the cloning vectors: plasmids and bacteriophages. ![]() Quantitative PCR (real-time PCR). Applications. Unit 3. Polymer chain reaction (PCR) Introduction. Southern, Northern blot and their applications. Basic Tools of Recombinant DNA: restriction enzymes, polymerases, exonuclases, ligases, reverse transcriptase. Introduction od recombinant DNA technology.
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